2 edition of study of cyanobacterial toxins by means of tandem mass spectrometry found in the catalog.
study of cyanobacterial toxins by means of tandem mass spectrometry
Vanessa Cordelia Meriel Dale
Thesis (Ph.D.) - University of Warwick, 1994.
|Statement||Vanessa Cordelia Meriel Dale.|
Cyanobacteria represent a bacterial phyllum characteristic by the ability to photosynthesize. They are potentially applicable for the production of useful compounds but may also cause poisoning or at least health problems as they can produce cyanotoxins. The introduction of a fast methodology is important not only for fundamental taxonomic purposes, but also for reliable identifications in Author: Marek Šebela, Eva Jahodářová, Martin Raus, René Lenobel, Petr Hašler. Cyanobacteria, commonly referred to as blue-green algae, are a phylum of photosynthetic bacteria that are ubiquitous in freshwater, estuarine and marine waters .Many of the genera in this phylum are positively buoyant and, when conditions are appropriate, they can “bloom” as dense, paint-like scums on the water surface .Global climate change, particularly Cited by: Microbore liquid chromatography and positive ion electrospray mass spectrometry are applied to the determination of 16 carbamate, urea, and thiourea pesticides and herbicides in water. The electrospray mass spectra of the analytes were measured and are discussed and mobile-phase matrix effects were evaluated. Analyte positive ion abundances Cited by: The neurotoxin β-N-methylamino-L-alanine (BMAA) produced naturally by cyanobacteria, diatoms and dinoflagellates can be transferred and accumulated up the food chain and may be a risk factor for Cited by:
Astronomers use mass spectrometry to determine the elements and isotopes found in the solar wind. For example, the mass spectrum of solar wind reveals that the following elements are common: carbon (12 amu), oxygen (16 amu), neon (20 amu), magnesium (24 amu), silicon (28 amu) and iron (56 amu).
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Analysis of Microcystins by Online Solid Phase Extraction–Liquid Chromatography Tandem Mass Spectrometry (Pages: ) Cintia Flores; Josep Caixach. Request PDF | Detailed Study of Cyanobacterial Microcystins Using High Performance Tandem Mass Spectrometry | Microcystins (MC) are a large group of toxic cyclic peptides.
High‐Resolution Mass Spectrometry (HRMS) MS Experiments for the Detection of Unknown Cyanotoxins Performance Criteria of LC‐MS Methods for Identification and Quantification of Cyanotoxins References 24 Capillary Electrophoresis of Cyanobacterial Toxins Gábor Vasas.
Microcystins (MC) are a large group of toxic cyclic peptides, produced by cyanobacteria in eutrophic water systems.
Identification of MC variants mostly relies on liquid chromatography (LC) combined with collision-induced dissociation (CID) mass by: Study of cyanobacterial toxins by means of tandem mass spectrometry book of Cyanobacterial Toxins by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry Chapter in Journal of Chromatography A (1).
The description of toxin analysis methods in this chapter includes an introduction to toxins, sample preparation, and mass spectrometry followed by specific methods for the analysis of saxitoxin, α-amanitin, botulinum neurotoxins (BoNT), and ricin using mass by: 2.
Risk Assessment of Toxic Cyanobacterial Outbreaks in Water for Human Consumption in Italy Framework of Risk Management of Toxic Cyanobacterial Study of cyanobacterial toxins by means of tandem mass spectrometry book in Water for Human Consumption Risk Information and Communication References Section IV Toxins and Bioactive/Noxious Compounds from Cyanobacteria In this study, we have developed a new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for fast multiresidue determination of five toxins in suspended material and sediment samples.
The combination of hydrophilic interaction liquid chromatography with electrospray mass spectrometry (HILIC–MS) has been investigated as a tool for the analysis of assorted toxins produced by cyanobacteria. Toxins examined included saxitoxin and its various analogues (1–18), anatoxin-a (ATX-a, 19), cylindrospermopsin (CYN, 20), deoxycylindrospermopsin (doCYN, 21), and microcystins-LR Cited by: Drastic environmental conditions such as elevated temperature, abrupt pH study of cyanobacterial toxins by means of tandem mass spectrometry book, low turbulence, and high nutrient inputs can enhance the developme Cited by: The precision of the liquid chromatography–tandem mass spectrometry (LC-MS/MS) method is expressed as the relative standard deviation (RSD).
Intraday RSD results are for a 1-day study (n ¼ 3. Cyanotoxins From Water. Cyanotoxins are toxins produced by cyanobacteria; the organisms were previously called blue-green algae. Information about the toxicology of cyanotoxins may be found in the chapter on cyanobacterial (blue-green algae) toxins elsewhere in this book.
Hiller, B. Krock, A. Cembella, B. LuckasRapid detection of cyanobacterial toxins in precursor ion mode by liquid chromatography tandem mass spectrometry Journal of Mass Spectrometry, 42 (9) (), pp. Cited by: tandem mass spectrometry (LC-MS/MS) method for fast multiresidue determination of five toxins in suspended mate-rial and sediment samples.
For each target compound, two. Nine lakes (Garda, Maggiore, Como, Iseo, Lugano, Idro, Pusiano, Ledro, and Levico) located in the Italian subalpine lacustrine district were chosen for a comparative study of the diversity and distribution of cyanobacterial toxins. All nine lakes are known to host different toxic cyanobacteria, mainly Planktothrix rubescens.
An analytical Cited by: Handbook of cyanobacterial monitoring and cyanotoxin analysis. Cyanobacteria 13 Spatial Distribution of Cyanobacteria in Freshwater Ecosystems 15 Ecology of the Production of Toxins by Cyanobacteria 16 General Conclusions 17 References 17 3 Picocyanobacteria: The Smallest Cell-Size Cyanobacteria 19Iwona Jasser and Cristiana.
Microcystins, anatoxins and okadaic acid are toxins produced by freshwater cyanobacteria and marine dinoflagellates. These toxins have been the responsible for the illness and death of biota and humans.
To determine their presence in water during blooms, sensitive analytical methods are needed. In this study, we have developed a new liquid chromatography tandem mass spectrometry Cited by: 3.
In this study, fish specimens considered as potential vectors of CTXs were caught in Madeira and Selvagens archipelagos for toxins determination via sensitive liquid chromatography with tandem mass spectrometry detection (LC–MS/MS).
CTXs were found in most of the fish samples from Selvagens and none from Madeira. The effectiveness of tandem mass spectrometry in structural elucidation of cyanobacterial peptides has been demonstrated in numerous papers, e.g., [3,4,12,13,14].
The LC-MS/MS method appears to be especially useful in the analysis of small amounts of compounds in complex natural by: An intercollaborative study was organized to evaluate the performance characteristics of a liquid chromatography tandem mass spectrometry procedure for the simultaneous determination of 12 mycotoxins in food, which were ochratoxin A, aflatoxins B1, B2, G1, G2, and M1, deoxynivalenol, zearalenone, fumonisins B1 and B2, and T-2 and HT-2 toxins.
Dolphin stranding events occur frequently in Florida and Massachusetts. Dolphins are an excellent sentinel species for toxin exposures in the marine environment. In this report we examine whether cyanobacterial neurotoxin, β-methylamino-L-alanine (BMAA), is present in stranded dolphins.
BMAA has been shown to bioaccumulate in the marine food web, including Cited by: 3. Abstracts. A general approach to the identification of cyanobacterial peptides is considered.
It is based on a combination of liquid chromatography high resolution tandem mass spectrometry with the prior by: 5. New measurements of cyanobacterial toxins in natural waters using high performance liquid chromatography coupled to tandem mass spectrometry. Hedman CJ(1), Krick WR, Karner Perkins DA, Harrahy EA, Sonzogni WC.
Author information: (1)Wisconsin State Lab. of Hygiene, Agriculture Drive, Madison, WI,by: this study, an analytical method has been developed using Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) for the analysis of saxitoxins from spirulina supplements and cyanobacterial mass.
Target saxitoxins were Saxitoxin, decarbamoylsaxitoxin, Neosaxitoxin, Gonyautoxin 2&3, decarbamoylgonyautoxin 2&3. Shark fins and cartilage also contain β- N -methylamino- l -alanine (BMAA), a ubiquitous cyanobacterial toxin linked to neurodegenerative diseases.
Today, a significant number of shark species have found their way onto the International Union for Conservation of Nature (IUCN) Red List of Threatened by: 7.
Cyanobacterial Toxins in Drinking Water Document for Public Consultations. Science and Technical Considerations.
The Federal-Provincial-Territorial Committee on Drinking Water (CDW) has assessed the available information on cyanobacterial toxins with the intent of establishing a drinking water guideline. The purpose of this consultation is to. Cyanobacteria are widely distributed in fresh, brackish, and ocean water environments, as well as in soil and on moist surfaces.
Changes in the population of cyanobacteria can be an important indicator of alterations in water quality. Metabolites produced by blooms of cyanobacteria can be harmful, so cell counts are frequently monitored to assess the potential risk from cyanobacterial by: 2.
The liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses provided evidence that both blue mussels collected after a cyanobacterial bloom in the Baltic Sea and the crustaceans exposed under laboratory conditions to N.
spumigena extract accumulated the cyclic anabaenopeptins (APs). In the crustaceans, the linear peptides, spumigins. Anabaenopeptins, bioactive cyclic hexapeptides, were isolated by preparative reversed-phase high performance liquid chromatography from an extract of Baltic Sea cyanobacterial bloom material composed of Nodularia spumigena (50%), Aphanizomenon flos-aquae (40%) and Dolichospermum spp.
(10%). Five new anabaenopeptins and nine previously known Cited by: In this study, we used a mass spectrometry based approach to digitize (convert to data format that can be stored, shared and analyzed by computational tools) the chemical inventory of an established marine cyanobacteria and algae collection in order to better evaluate its diversity and probe for novel natural by: Microcystins Analysis Methods Ap Heather Raymond apart) cyanobacteria cells and release toxins.
• Labs must demonstrate they can achieve an acceptable level of precision tandem Mass Spectrometry. Data Reporting Consideratio ns • Specify Analysis Method.
Optimizing recoveries of two chlorotriazine herbicide metabolites and 11 pesticides from aqueous samples using solid-phase extraction and gas chromatography–mass spectrometry. Journal of Chromatography A(), DOI: /S(97)Cited by: In the present study, a hydrophilic interaction liquid chromatographic-tandem mass spectrometric method with a lower limit of detection of fg on column (signal-to-noise ratio = 3, n = 9) and a lower limit of quantification of 1 pg on column (signal-to-noise ratio = 11, n = 9) with demonstrated application in 4 aquatic organisms is by: 6.
Toxins – Testai E, Buratti FM, Funari, E et al., Review and analysis of occurrence, exposure and toxicity of cyanobacteria toxins in food. EFSA Supporting Publication ; 13(2):EN–, pp. WHO () Cyanobacterial toxins: Microcystin-LR in.
Interests: marine algal, bacterial and cyanobacterial toxins, mass spectrometric analysis of and biological which showed differences in PnTx-G toxic potency.
Thus, an acute toxicity study was carried out at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. Risk Assessment of Toxic Cyanobacterial Outbreaks in Water for Human Consumption in Italy Framework of Risk Management of Toxic Cyanobacterial Outbreaks in Water for Human Consumption Risk Information and Communication References Section IV Toxins and Bioactive/Noxious Compounds from Cyanobacteria Brand: Wiley.
2. Results and Discussion. The fins of seven shark species collected in South Florida coastal waters (Table 1) were analyzed by high performance liquid chromatography with fluorescence detection (HPLC-FD).BMAA was detected in a total acid hydrolysate using HPLC-FD and validated by triple quadrupole liquid chromatography tandem mass spectrometry Cited by: Oehrle S, Westrick J.
Detection of various freshwater cyanobacterial toxins using ultra performance liquid chromatography-tandem mass spectrometry, emerging aspects on freshwater harmful cyanobacterial blooms and their effects on drinking water quality.
Presented at the U.S. EPA Office of Research and Development, Cincinnati, OH, Novem This Health Advisory (HA) for the cyanobacterial toxin cylindrospermopsin is focused on drinking water as the primary source of exposure.
Exposure t o cyanobacteria and their toxins may also occur by ingestion of toxin-contaminated food, including consumption of fish, and by. Determination of microcystins by mass spectrometry: Unambiguous identification of microcystins may be achieved with a mass spectrometry (MS) detector, ideally with tandem mass spectrometry (MS-MS) in order to obtain information both on the molecular signal and fragments.
Detection limits of µg/l and less can be achieved. The extract was centrifuged for 10 min and filtered through a μm pdf size filter to remove particulate matter. Accurate mass measurements were performed using LC/MS and liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis (Volkmann et al., ; Volkmann, ).
The extract was diluted to fold with the by: form or by any means, electronic, mechanical, photocopying, recording or otherwise, except as permitted by law. Liquid Chromatography–Mass Spectrometry (LC-MS) and Liquid Chromatography–Tandem Mass Spectrometry Other Algal and Cyanobacterial Toxins.
Dell’Aversano C, Eaglesham GK, Quilliam Ebook () Analysis of cyanobacterial toxins by hydrophilic interaction liquid chromatography–mass spectrometry. Journal of Chromatography A. – Accessed Jan Bumke-Vogt C, Mailahn W, Chorus I () AID-TOX15>CO;2-V/abstract. Accessed Jan Cited by: